Environmental Regulation of Salmonella typhi Invasion-Defective Mutants
نویسندگان
چکیده
منابع مشابه
Environmental regulation of Salmonella typhi invasion-defective mutants.
Salmonella typhi is the etiologic agent of human typhoid. During infection, S. typhi adheres to and invades epithelial and M cells that line the distal ileum. To survive in the human host, S. typhi must overcome numerous complex extracellular and intracellular environments. Since relatively little is known about S. typhi pathogenesis, studies were initiated to identify S. typhi genes involved i...
متن کاملIntact motility as a Salmonella typhi invasion-related factor.
Invasiveness of Salmonella typhi was investigated. At first, we introduced Tn5 into the chromosome of a wild-type S. typhi strain, GIFU 10007, and screened the independent Tn5 insertion mutants for noninvasive (Inv-) strains. During the first half of this work, we obtained 4 Inv- strains from 1,338 independent Tn5 mutants. The four were either nonflagellate (Fla-), nonmotile (Mot-), or nonchemo...
متن کاملSalmonella typhimurium mutants defective in cytidine monophosphate kinase (cmk).
Mutants of Salmonella typhimurium defective in cytidine 5'-monophosphate (CMP) kinase (cmk) have been isolated. The mutants also lack the ability to phosphorylate 2'-deoxyCMP, indicating that one enzyme is responsible for the phosphorylation of both CMP and deoxyCMP to the corresponding diphosphates. In glucose minimal medium the mutants grow at the same rate as the parental strain; however, th...
متن کاملSalmonella typhimurium prfA mutants defective in release factor 1.
Mutations have been characterized that map in the prfA gene of Salmonella typhimurium. These weak amber suppressors show increased readthrough of UAG but not UAA or UGA codons. Some hemA mutants exhibit a similar suppressor activity due to transcriptional polarity on prfA. All of the suppressors mapping in prfA are recessive to the wild type. Two mutant prfA genes were cloned onto plasmids, and...
متن کاملOsmolarity and growth phase overlap in regulation of Salmonella typhi adherence to and invasion of human intestinal cells.
The study of the effects of osmolarity and growth phase on Salmonella typhi adherence to and invasion of Henle 407 epithelial cells provides the first evidence of a clear overlap between these two environmental stimuli. High-osmolarity conditions are required in the late-log phase for optimum induction of the adherent and invasive phenotypes.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1998
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.66.2.682-691.1998